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. 2017 Jul 5;2017:8492797. doi: 10.1155/2017/8492797

Figure 4.

Figure 4

WJ-MSC licensed with IFN-γ inhibit CD3+ T cell proliferation. WJ-MSC were seeded, and IFN-γ was added for 24 hours. PBMC were stimulated with PHA for 3 days in the presence of WJ-MSC; the ratio used was 1 : 10 (WJ-MSC : PBMC). The WJ-MSC passages used in the experiments were P5 and P10. T cells were collected, stained with anti-CD3, anti-CD4, and anti-CD8 antibodies, and proliferation was measured by flow cytometry using the Click It Kit. The percentage of inhibition (Inhib) was calculated using the percentage of proliferation of PBMC + PHA + WJ-MSC compared to the control PBMC + PHA. Experiments were performed in triplicate. Results are represented by mean ± SD. Statistically significant differences are shown as ∗p < 0.05, ∗∗p < 0.005, and ∗∗∗p < 0.0001 (n = 3).