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. 2017 Jul 5;2017:8492797. doi: 10.1155/2017/8492797

Figure 5.

Figure 5

Gene expression of IL-10 and IDO is increased in WJ-MSC after coculture with PBMC. WJ-MSC were seeded, after 24 hours. PBMC was added and stimulated with PHA for 3 days in the presence of MSC; the ratio used was 1 : 10 (MSC : PBMC). The WJ-MSC passages used in the experiments were P5 and P10. After 3 days, WJ-MSC were lysed, total RNA extracted, and real-time PCR performed. (a) IDO and (b) IL-10 gene expression of WJ-MSC1, WJ-MSC2, and WJ-MSC3. Gene expression was normalized by GAPDH and expressed as fold change compared to the control WJ-MSC. Experiments were performed in triplicate. Results are represented as mean ± SD. Statistically significant differences are shown as ∗p < 0.05, ∗∗∗p < 0.0001 (n = 3).