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. 2015 Dec 10;1:15016. doi: 10.1038/npjsba.2015.16

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Copyright © 2015 The Systems Biology Institute/Macmillan Publishers Limited

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Cdc14 endocycles. (a) When grown in galactose, GAL-CLB2-dbΔ cells express a mutant form of Clb2 that is not degraded by APC:Cdc20 and only weakly degraded by APC:Cdh1 (37% of that for the wild-type protein). Because these cells maintain a high level of Clb2-kinase activity, they cannot exit mitosis, and yet they exhibit regular oscillations of Cdc14 release and resequestration (period=73 min). (b) The frequency of Cdc14 endocycles depends linearly on Clb2 level. (c,d) Similar oscillations in Cdc14 release are exhibited by the GAL-CLB2^nd strain. The CLB2^nd allele encodes Clb2 protein that is not degraded by either APC:Cdc20 or APC:Cdh1. The simulations in this figure agree well with experimental observations in refs 7,64.