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. 2016 Sep 8;2:16017. doi: 10.1038/npjsba.2016.17

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Proton-dependent reactions altered by the pH shift experiment as implemented in the genome-scale model. The state at pH 7.5 is highlighted in blue, pH 6.5 in yellow. R_PIt6/R_PIht6, phosphate transport via H⁺ symport at pH 7.5 and 6.5, respectively; R_LACLt_pH7.5/R_LACLt, L-lactate transport via H⁺ symport at pH 7.5 and 6.5, respectively; R_ATPS3r, V-type H⁺ translocating ATPase. The pH-dependent flux boundaries for these reactions are listed in Supplementary Table S8.