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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Cancer Res. 2017 May 18;77(13):3467–3478. doi: 10.1158/0008-5472.CAN-17-0056

Figure 2. Genes that undergo methylation in tumors have reduced expression at the time point of highest oxidative damage.

Figure 2

A) 8-oxoG dot blot of DNA extracted from distal colon epithelium from mock or ETBF-inoculated mice, N=3. Bar graph of densitometry determined using ImageJ for dots. Mean +/− SEM. B) 8-oxoG staining by flow cytometry of Epcam-positive cells from mice treated as in A. C) Expression of candidate genes by qPCR in distal colon epithelium from mice at the indicated days post-mock or ETBF. Mean +/− SEM. N=5. *p<0.05. D) EZH2, IgG and E) H3K27me3 enrichment by ChIP relative to input at promoters of indicated genes in distal colon epithelium from mice 2 days post-mock or ETBF. Mean +/− SEM. N=3. *p<0.05. See also Figure S2.