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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2017 May 25;37(7):1339–1351. doi: 10.1161/ATVBAHA.117.309021

Figure 6. NF-κB controls the activation of nuclear factor of activated T cells complex 1 (NFATc1).

Figure 6

A–C VICs from normal aortic valves were treated with Bay11-7082 (10 μM) or SN50 (100 μg/ml) 1 h prior to matrilin2 (2.0 μg/ml) stimulation for 48 h. Immunoblots and densitometric data show that either Bay11-7082 or SN50 failed to suppress matrilin2-induced expression of NFATc1 (A and B). NFATc1 activity data show that inhibition of NF-κB by Bay11-7082 or SN50 attenuated NFATc1 activation induced by matrilin2 (C). D. Immunoblots for NF-κB p65 and NFATc1 after immunoprecipitation with anti-p65 antibody, anti-NFATc1 antibody or non-immune IgG demonstrate interaction of these two nuclear factors. IP, immunoprecipitation; IB, immunoblotting. Mean ± SE; n=4 separate experiments using distinct cell isolates; *P<0.05 vs. control; #P<0.05 vs. matrilin2 + DMSO or matrilin2 + SN50M; NS = non-significant.