Figure 3.

Chemotactic responsiveness of bone marrow-derived mononuclear cells (BM-MNCs) to SDF-1 and S1P gradients and of Gr-1⁺ cells and monocytes to SDF-1, S1P, and promobilizing C5a and _desArgC5a chemoattractant gradients. The chemotactic responsiveness of murine clonogenic colony-forming unit-granulocyte/macrophage (CFU-GM) progenitors to stromal cell-derived factor 1 (SDF-1) and sphingosine 1-phosphate (S1P) gradients (A). Gr-1⁺ cells (B) and monocytes (C) were employed for chemotaxis assays in response to S1P, SDF-1, complement component 5a (C5a), and _desArgC5a gradients using the Transwell system. The experiment was repeated twice, and the data were combined. Results are shown as the percentage of input which represents 5% of the insert (*p ≤0.05).