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. Author manuscript; available in PMC: 2017 Jul 19.
Published in final edited form as: J Nucl Med. 2008 Jun;49(Suppl 2):96S–112S. doi: 10.2967/jnumed.107.045948

FIGURE 11.

FIGURE 11

Comparison of expression of CMV-mtfl-mrfp1-wttk and CMV-mtfl-jred-wttk vectors by fluorescence and BLI in living mice. (A) Two and 4 million 293T cells were transiently transfected with CMV-mtfl-jred-wttk (left) and CMV-mtfl-mrfp1-wttk (right) vectors, respectively, and implanted on dorsal side of nude mouse (sites 1 and 2 have 2 million cells and sites 3 and 4 have 4 million cells) and imaged for fluorescence using Maestro system. Fluorescence signal from cells expressing CMV-mtfl-mrfp1-wttk was clearly visible (2 and 4). However, cells expressing CMV-mtfl-jred-wttk plasmid (1 and 3) show faint fluorescence signal. (B) Same mouse was then injected with D-luciferin and imaged for bioluminescence in Xenogen IVIS 200 optical system. Bioluminescence signal showed equal signal intensity by both groups of cells (2 and 4 vs. 1 and 3). (Adapted with permission of (183).)