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. Author manuscript; available in PMC: 2017 Jul 19.
Published in final edited form as: J Nucl Med. 2008 Jun;49(Suppl 2):96S–112S. doi: 10.2967/jnumed.107.045948

FIGURE 4.

FIGURE 4

(A) Representative functional MRI maps of pooling and draining voxels for MDA-MB-231 and MCF-7–bearing animals. (B) Box-and-whisker plot comparing volume of extravasated fluid pooled or drained between MDA-MB-231 and MCF-7 xenografts (*P < 0.1 with 1-sided Mann–Whitney U test). (C) Phase contrast image obtained at ×40 from 5-μm-thick hematoxylin-stained MDA-MB-231 tumor section overlaid with same tumor section stained with LYVE-1 (lymphatic vessel marker), with transparency of latter adjusted to enable visualization of lymphatics packed with tumor cells. (D) Photomicrograph obtained at ×40 from 5-μm-thick hematoxylin- and eosin-stained axillary lymph node from MDA-MB-231–bearing animal, scored positive for presence of cancer cells (arrows). (E) Photomicrograph obtained at ×40 from 5-μm-thick hematoxylin- and eosin-stained lung from MDA-MB-231–bearing animal scored positive for presence of cancer cells (arrows). (F) Similar image obtained at ×40 from 5-μm-thick hematoxylin-stained MCF-7 tumor section overlaid with image of same tumor section stained with LYVE-1, with transparency of latter adjusted to enable visualization of lymphatics that have few, if any, tumor cells. (G) Photomicrograph obtained at ×40 from 5-μm-thick hematoxylin- and eosin-stained axillary lymph node from MCF-7–bearing animal, scored negative for presence of cancer cells. (H) Photomicrograph obtained at ×40 from 5-μm-thick hematoxylin- and eosin-stained lung from MCF-7–bearing animal scored negative for presence of cancer cells. (Adapted with permission of (76).)