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. Author manuscript; available in PMC: 2017 Jul 19.
Published in final edited form as: Biochemistry. 2016 Dec 20;56(1):179–188. doi: 10.1021/acs.biochem.6b00884

Table 2.

Specific activity and activity ratio measurements for different GSY2 mutants. Data are represented as mean ± SEM.

Sp Activity (μmole/min.mg) (-G-6-P) Sp activity (μmole/min.mg) (7.2mM G-6-P) Activity ratio (-G-6-P/+G-6-P)
WT Gsy2 1.03±0.03 1.73±0.03 0.6±0.01
Gsy2-R580A/R581A/R583A1 0.55±0.02 0.51±0.03 1.09±0.02
Gsy2-R587A/R589A/R592A1 0.38±0.01 0.35±0.02 1.09±0.01
Gsy2(1-640) +49mer T668P1 0.1±0.01 1.9±0.02 0.05±0.01
Gsy2-R589A/R592A 0.1±0.01 1.56±0.02 0.11±0.01
Gsy2-R581C/R592C 0.033±0.001 0.04±0.001 NA
Gsy2-R581C/R592C +200mM BME 0.58±0.01 2.1±0.02 0.27±0.02
Gsy2-R581A/R592A 0.41±0.02 1.88±0.04 0.28±0.03
Gsy2(1-640)-R589A/R592A + 49mer T668 0.03±0.01 0.03±0.01 NA
1

Data are from reference 16.