Fig. 5.

Efflux pump inhibition assay data for 3,4-dimethoxy-2-(methoxycarbonyl)benzoic acid (1) and 3,5,3′-trihydroxy-7,4′-dimethoxy-6,8-C-dimethyl-flavone (2). The positive control for this assay is carbonyl cyanide m-chloro-phenylhydrazone (CCCP), a compound that inhibits efflux by collapsing the proton motive force across the cell membrane (Hopfer et al., 1967). Each data point is the mean of triplicate measurements from separate bacterial cultures (biological replicates) and error bars represent standard error of the mean.