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. Author manuscript; available in PMC: 2017 Dec 6.
Published in final edited form as: Anal Chem. 2016 Nov 15;88(23):11900–11907. doi: 10.1021/acs.analchem.6b03725

Figure 2.

Figure 2

ADEPT and CUSUM+ analysis applied to a simulated nanopore measurement (gray). Two events with the same current blockade (red) but different residence times (tres), with respect to the system characteristic relaxation time (τ) are shown. (Left) For a long event (tres ≥ 5τ), the ionic current converges close to its steady-state value, and the current levels estimated by ADEPT and CUSUM+ are equivalent. (Right) For short events (e.g., tres ≈ 2τ), the current does not reach the steady-state value of the idealized pulse (red). In this case, CUSUM+ and other algorithms used in nanopore analysis systematically underestimate the steady-state current (blue) by an amount Δi (gray; dashed). In contrast, the physical model underlying ADEPT allows the algorithm to accurately estimate an event’s steady-state current.