Table 3. List of the host proteins which were shown to have significantly increased expression (≥ 2-fold) during disease in rabbits infected with Encephalitozoon cuniculi.
| Protein identification | Foldchange | Known function | Chromosome | Gene name (GenBank®) | Access.number (UniProt®) | Subcellularlocation | Theoretical MW (kDa) | Calc.pI | Proteinscore (Sequest®) | No.uniquepeptide(s) | No.aa | % Seq.coverage |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Alpha-1-antiproteinase F precursor | 2.1 | metabolic process (catalytic activity) | LOC100008973 | G1TFV7 | extracellular space | 45.8 | 6.2 | 508 | 2 | 413 | 48 | |
| Alpha-1-antiproteinase S-1 | 2.4 | metabolic process (catalytic activity) | LOC100008980 | Q07298 | extracellular space | 45.7 | 6.4 | 521 | 1 | 413 | 46 | |
| Ig gamma-chain C region | 2.5 | defense response and transport (protein binding) | LOC100009097 | G1THZ6 | extracellular space | 51.9 | 7.7 | 104 | 2 | 480 | 12.6 | |
| Haptoglobin | 2.4 | metabolic process (catalytic activity); transport (protein binding); regulation of biological process; response to stimulus | V | HP | G1SWF6 | extracellular region | 38.9 | 6.3 | 171 | 12 | 347 | 29.7 |
| Serum paraoxonase/ arylesterase | 2.6 | metabolic process (catalytic activity); transport (metal-ion binding, protein binding); response to stimulus; regulation of biological process | X | PON1 | G1SN96 | blood microparticle; extracellular; spherical high-density lipoprotein particle | 38.2 | 5.6 | 113 | 1 | 342 | 38 |
| Cystatin fetuin-B-type | 2.6 | regulation of biological process (enzyme regulator activity) | XIV | FETUB | G1SEK8 | extracellular exosome; extracellular space | 42.9 | 5.9 | 146 | 7 | 392 | 15 |
| Ceruloplasmin | 2.0 | metabolic process (catalytic activity); transport (metal-ion binding); cellular homeostasis | XIV | CP | G1SJX3 | cell | 121.1 | 5.6 | 164 | 20 | 1067 | 24 |
| Hemoglobin subunit beta-1/2 | 3.1 | transport (transporter activity, metal ion binding) | I | HBB1 / HBB2 | P02057 / A0A140TAV6 | hemoglobin complex | 16.1 | 8.2 | 456 | 4 | 147 | 79 |
| Alpha-hemoglobin | 2.4 | transport (transporter activity, metal ion binding) | HBA | B8K132 | hemoglobin complex | 15.6 | 8.2 | 208 | 1 | 142 | 51 |
Fold changes in the 31 diseased rabbits (i.e. encompassing all the subjects for which blood samples were tagged with either 116- or 117-labels) were calculated with respect to the protein expression in the 35 non-diseased rabbits undergoing non-E. cuniculi inflammation due to any miscellaneous cause (114-tag reagent).
Abbreviations: aa, Amino acids; Access., Accession; calc., Calculated; kDa, Kilo dalton; MW, Molecular weight; No, Number; pI, Isoelectric point; Seq., Sequence