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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Dig Dis Sci. 2016 Apr 13;61(8):2252–2261. doi: 10.1007/s10620-016-4145-y

Fig. 3.

Fig. 3

Inhibition of nmMLCK results in attenuated accumulation of cytosolic claudin-3. (A) Caco2 cells were treated with vehicle control or ML-7 (10 μM) followed by IL-1β treatment and claudin-3 protein localization was assessed by immunofluorescent confocal microscopy. Quantitation represents (B) the ratio of intensity of claudin-3 (green) detection at the cell-cell border (blue arrows) vs. cytosolic expression (white arrows), (C) the ratio of E-cadherin intensity at cell-cell borders (blue arrows) vs. cytosolic expression (white arrows), and (D) the percentage of cytosolic claudin-3 or E-cadherin from total intensity for each treatment. Results are representative of 3 experiments. * p<0.05 relative to vehicle control, ¥ p<0.05 relative to IL-1β alone.