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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Anal Bioanal Chem. 2017 Mar 29;409(13):3417–3427. doi: 10.1007/s00216-017-0285-y

Fig. 3.

Fig. 3

HPLC chromatograms derived from TBOP alone (50 µM) before the reaction of TBOP with BSA (A); reaction mixture of TBOP with BSA (1:10) at room temperature in a Tris buffer (0.1 M, pH 7.4) for 20 min (B); reaction mixture of TBOP with GSH (1:10) at room temperature in a Tris buffer (0.1 M, pH 7.4) for 20 min (C); BSA alone (D). TBOP alone (50 µM) before the reaction of TBOP with protein precipitates from cell homogenates (E); reaction mixture of TBOP with the protein precipitates (F). The HPLC condition is presented in the experimental section except the mobile phase. The mobile phase included solvent A (acetonitrile) and solvent B (0.1% trichloroacetic acid aqueous solution). The initial 2 min was solvent B with 10% solvent A. Solvent A was then linearly increased to 70% in 11 min, and then further increased to 90% in 5 min and kept at 90% for an additional 3 min before back to 10% in 2 min.