Table 1.

NPSH quantified by the TBOP imaging assay vs a reported GUALY’s reagent plate reader method.

NEM concentration (µM)	0 (control)	3	4	5	6	7	8	9	10
NPSH determined by TBOP imaging*	100.00% ±8.90%	79.65% ±4.76%	86.33% ±8.51%	70.22% ±8.43%	63.13% ±5.10%	57.62% ±9.35%	49.36% ±4.91%	44.05%± 11.13%	28.93% ±5.25%
NPSH determined by GUALY’s reagent**	100.00% ±4.09%	83.29% ±1.50%	83.57% ±2.11%	81.69% ±6.10%	66.64% ±2.52%	71.53% ±7.93%	54.88% ±4.01%	47.57%± 3.81%	33.55% ±5.57%

^*n=6 spots from the same sample.

^**n=3 wells from the same plate. Data are presented as the percentage of control in which cells were treated with no NEM. Data are from one representative experiment of a triplicate.