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. Author manuscript; available in PMC: 2017 Jul 19.
Published in final edited form as: Mol Microbiol. 2004 May;52(3):801–814. doi: 10.1111/j.1365-2958.2004.04016.x

Fig. 5.

Fig. 5

Quantification of EzrA protein in B. subtilis. Wild-type cells (JH642) grown to mid-exponential phase (OD600 = 0.5) in Luria broth (LB) were sampled for colony-forming units and lysed as described in Experimental procedures. Different amounts of lysate (6.29 × 103 cfu μl−1) were loaded in lane 1 (5 μl) and lane 2 (10 μl). EzrA was detected by immunoblotting with whole sera. EzrA was quantified by comparing the intensity of the bands in lanes 1 and 2 with that of a twofold serial dilution of purified EzrA fusion protein (four right hand lanes) taking into account the number of colony-forming units. Based on these data, we estimate that there are 10 000–20 000 copies of EzrA per cell during exponential growth in LB.