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. Author manuscript; available in PMC: 2018 Apr 24.
Published in final edited form as: Dev Cell. 2017 Apr 24;41(2):129–142.e6. doi: 10.1016/j.devcel.2017.02.006

Figure 4. Hyperphosphorylation Reduces the Transcriptional Activity of NEUROG3.

Figure 4

(A) PAX4 minienhancer-driven luciferase transcription is reduced by NEUROG3 phosphorylation. HeLa cells were cotransfected with the mini-enhancer, an Hnf1α overexpression construct, and FLAG-tag control (FLAG) or WT or mutant forms of NEUROG3.

(B) mPAC cells were transfected with FLAG-tag control, WT-NEUROG3, or the indicated mutants and analyzed for expression of endogenous Pax4 by qPCR.

(C) mPAC cells were transfected with FLAG-tag control, WT-NEUROG3, or the indicated mutants and analyzed for expression of endogenous NeuroD1 by qPCR.

(D) mPAC cells were transfected with FLAG-tag control, WT-NEUROG3, or the indicated mutants and analyzed for expression of endogenous Somatostatin (Sst) by qPCR.

Data are presented as mean ± SEM. n = 3. *p < 0.05 by one-way ANOVA and Dunnett’s post hoc test. See also Figures S3 and S4.

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