REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Donkey polyclonal Anti-mouse IgG	Jackson ImmunoResearch	Cat#715-001-003; RRID: AB_2307338
Goat polyclonal anti-Cpa1	R&D systems	Cat#AF2765; RRID: AB_2085841
Guinea Pig anti-NEUROG3	Schwitzgebel et al., 2000	German Lab
Guinea Pig polyclonal anti-Insulin	DAKO	Cat#A056401-2; RRID: AB_2617169
Mouse monoclonal anti-FLAG	Sigma-Aldrich	Cat#F3165; RRID: AB_259529
Mouse monoclonal anti-Glucagon	Sigma-Aldrich	Cat#G2654; RRID: AB_259852
Mouse monoclonal anti-Neurog3	Developmental Studies Hybridoma Bank	Cat#F25A1B3; RRID: AB_528401
Mouse monoclonal anti-Pdx1	Developmental Studies Hybridoma Bank	Cat#F109-D12; RRID: AB_1157903
Rabbit polyclonal anti-ChromograninA	Thermo Scientific	Cat#RB-9003-P1; RRID: AB_149730
Rabbit polyclonal anti-P27	Cell Signaling Technology	Cat#2552S; RRID: AB_10693314
Rabbit polyclonal anti-pHH3	Cell Signaling Technology	Cat#9701
Rabbit polyclonal anti-Sox9	Millipore	Cat#AB5535; RRID: AB_2239761
Rabbit polyclonal anti-β-actin	Abcam	Cat#AB8227; RRID: AB_2305186
Sheep polyclonal anti-NEUROG3	R&D systems	Cat#AF3444; RRID: AB_2149527
Biological Samples
CyT49 human embryonic stem cell (passages 20-p31)	Viacyte	CyT49; NIH hESC-10-0041
Human Peripheral Blood Mononuclear Cells	STEMCELL Technologies	Cat#70025.1
Chemicals, Peptides, and Recombinant Proteins
5′-Ethynyl-2′-deoxyuridine (EdU)	Sigma-Aldrich	Cat#T511285
Alexa Fluor 594 Carboxamido-(6-Azidohexanyl), Triethylammonium Salt	ThermoFisher Scientific	Cat#A10270
Ascorbate	Sigma-Aldrich	Cat#A4544
Accutase	STEMCELL Technologies	Cat#07920
Activin A	E-biosciences	Cat#34-8993-85
Alexa-Fluor 594 azide	ThermoFisher Scientific	Cat#A10270
BIX02189	Tocris Biosciences	Cat#4842
Bovine Serum Albumin	Sigma-Aldrich	Cat#A3803
Cdk2 inhibitor II	EMD Millipore	Cat#219445
Cdk2 inhibitor III	EMD Millipore	Cat#238803
CHIR-99021	Sigma-Aldrich	Cat#SML1046
Copper Sulfate	Sigma-Aldrich	Cat#451657
DAPI	Sigma-Aldrich	Cat#D9542
DMEM/F12	Corning	Cat#15-090-CV
Doxycycline	Sigma-Aldrich	Cat#D9891
FBS (GIBCO)	ThermoFisher Scientific	Cat#12483020
Gamma secretase inhibitor XX	EMD Millipore	Cat#565789
Geltrex	ThermoFisher Scientific	Cat#A1433302
Glutamax	ThermoFisher Scientific	Cat#35050061
Herugulin	PeproTech	Cat#100-03
Hoescht 33342	ThermoFisher Scientific	Cat#H1399
Horse Serum (GIBCO)	ThermoFisher Scientific	Cat#16050122
Hyclone D-PBS	GE Healthcare Bio-Science	Cat#SH30028.01
Hyclone DMEM	GE Healthcare Bio-Science	Cat#SH30081
Hyclone Penicillin/Streptomycin (P/S)	GE Healthcare Bio-Science	Cat#SV30010
Insulin-Transferrin-Selenum (ITS-X)	ThermoFisher Scientific	Cat#51500056
KGF	R&D Systems	Cat#251-KG
LDN193189	EMD Millipore	Cat#509882
Leptomycin B	Sigma-Aldrich	Cat#L2913
LY2228820	SelleckChem	Cat#S1491
MCDB 131	USBiological Life Sciences	Cat#E3000-01B
MG132	Cayman	Cat#1211877-36-9
Nocodazole	Sigma-Aldrich	Cat#31430-18-9
Paraformaldehyde	Sigma-Aldrich	Cat#P6148
PD-0332991	Sigma-Aldrich	Cat#PZ0199
Primestar DNA polymerase	Takara Clontech	Cat#R010A
Puromycin	Sigma-Aldrich	Cat#P8833
QuickExtract	Epicentre	Cat#QE09050
ReLeSR	STEMCELL Technologies	Cat#05872
ReproTESR	STEMCELL Technologies	Cat#05920
Repsox	Sigma-Aldrich	Cat#R0158
Restriction Enzymes	New England Biolabs	N/A
Retinoic Acid	Sigma-Aldrich	Cat#R2625
Roscovitine	Sigma-Aldrich	Cat#R7772
SANT-1	Tocris Biosciences	Cat#1974
SB216763	SelleckChem	Cat#S1075
Sodium Bicarbonate	Sigma-Aldrich	Cat#S6297
SP600125	Sigma-Aldrich	Cat#S5567
T3	Sigma-Aldrich	Cat#6397
TeSR-E8 Kit for hESC/hiPSC Matinenance	STEMCELL Technologies	Cat#05940
To-PRO-3 Iodide	ThermoFisher Scientific	Cat#T3505
Tris Base	Fisher Scientific	Cat#BP152
Tris HCl	Fisher Scientific	Cat#BP153
Triton X-100	Sigma-Adrich	Cat#T8787
Trizol	ThermoFisher Scientific	Cat#15596018
Vibrant DyeCycle Violet	ThermoFisher Scientific	Cat#V35003
XenoFree KOSR	ThermoFisher Scientific	Cat# A1099202
Zinc Sulfate	Sigma-Aldrich	Cat#Z0251
α-Amyloid Precursor Protein Modulator	EMD Millipore	Cat#565740
λ-phosphatase	New England Biolabs	Cat#P0753
Critical Commercial Assays
CytoTune-iPS 2.0 Sendai Reprogramming Kit	ThermoFisher Scientific	Cat#A16517
Dual Luciferase Assay	Promega	Cat#E1960
Erythroid Progenitor Reprogramming Kit	STEMCELL Technologies	Cat#05924
Lipofectamine 2000	ThermoFisher Scientific	Cat#11668027
Lipofectamine 3000	ThermoFisher Scientific	Cat#L3000015
QuickExtract DNA Extraction Solution	Epicentre	Cat#QE09050
Experimental Models: Cell Lines
Human: FUCCI-3: CyT49 FUCCI-3 transgenic hESC line	This paper	N/A
Human: N5-5: CyT49 Neurog3-2A-eGFP hESC line	This paper	N/A
Experimental Models: Organisms/Strains
Mouse: CD-1	Charles River	Crl:022
Mouse: Pdx1-Cre: B6.Cg-Tg(Pdx1-cre)89.1Dam/Mmucd	Mutant Mouse Regional Resource Centers	MMRRC:015970-UCD
Mouse: KrasLSL-G12D: B6.129S4-Krastm4Tyj/J	The Jackson Laboratory	JAX:008179
Mouse: TetO-Cdkn1b: C57BL/6(tetO-Cdkn1b)1Scpr/J	The Jackson Laboratory	JAX:017613
Mouse: Sox9-rtTA: C57BL/6-BAC-Sox9-rTTA	This paper	N/A
Recombinant DNA
Hnf1α expression plasmid	Smith et al., 2003	N/A
pCAGGS-FUCCI	This paper	N/A
pCCC	Krentz et al., 2014	N/A
pCCC-LL502/3	This paper	N/A
px458	Ran et al., 2013	Addgene #48138
Sequence-Based Reagents
Mouse Gusb (Mm00446953_m1)	ThermoFisher Scientific	Cat#4331182
Mouse Neurod1 (Mm01280117_m1)	ThermoFisher Scientific	Cat#4331182
Mouse Pax4 (Mm01159036_m1)	ThermoFisher Scientific	Cat#4331182
Mouse Sst (Mm00436671_m1)	ThermoFisher Scientific	Cat#4331182
Human NEUROD1 probe (FAM/ZEN/IaBKFQ): CGCCAGTTTCACCATTTCCGGG	IDT	N/A
Human NEUROD1 primer 1: TCCTTCGATAGCCATTCACATC	IDT	N/A
Human NEUROD1 primer 2: GCTGCCTTTTGTAAACACGAC	IDT	N/A
Human NEUROG3 gRNA: GGGTCGCTCCTCCAGCGACG	IDT	N/A
Human NEUROG3 probe (FAM/ZEN/IaBKFQ): AGCGGTCCTTCCCCAGAGC	IDT	N/A
Human NEUROG3 primer 1: TCTATTCTTTTGCGCCGGTAG,	IDT	N/A
Human NEUROG3 primer 2: GCAGGTCACTTCGTCTTCC	IDT	N/A
Human PDX1 probe (FAM/ZEN/IaBKFQ): CGC TTGTTCTCCTCCGGCTCC	IDT	N/A
Human PDX1 primer 1: TGAAGTCTACCAAAGCTCACG	IDT	N/A
Human PDX1 primer 2: GGAACTCCTTCT CCAGCTCTA	IDT	N/A
Neurog3-2A-eGFP PGK-Puro 3′ arm genotyping Forward primer: ATCAGCAGCCTCTGTTCCAC	IDT	N/A
Neurog3-2A-eGFP PGK-Puro 3′ arm genotyping Reverse primer: GGAGTCATCTTGCCAAGGCT	IDT	N/A
Neurog3-2A-eGFP PGK-Puro 5′ arm genotyping Forward primer: GGTAGAAAGGTAATATTTGGAGGCCT	IDT	N/A
Neurog3-2A-eGFP PGK-Puro 5′ arm genotyping Reverse primer: CTGAACTTGTGGCCGTTTACG	IDT	N/A
Software and Algorithms
CellProfiler	Carpenter et al., 2006	http://cellprofiler.org
NoD	Scott et al., 2010	http://www.compbio.dundee.ac.uk/www-nod/
NucPred	Brameier et al., 2007	http://www.sbc.su.se/~maccallr/nucpred/cgi-bin/single.cgi
SUMOsp	Xue et al., 2006	http://sumosp.biocuckoo.org/
WoLF PSORT	Horton et al., 2007	http://wolfpsort.org/