Skip to main content
. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Hum Pathol. 2016 May 31;56:40–51. doi: 10.1016/j.humpath.2016.05.014

Figure 4. Antibody detection of Dicer in nuclear and cytoplasmic fractions and by IHC.

Figure 4

A) T47D cell extracts showing Dicer expression in the nuclear versus cytoplasmic fraction with all three antibodies. TOPOI used as a control for nuclear protein. α-tubulin used as a control for cytoplasmic protein. B) T47D cells, FFPE and stained for Dicer with the three antibodies. Bar = 25μm. C) ER+ Breast cancer stained for Dicer with ab5818 alone (left) and with ab5818+10× blocking peptide (right). Bar = 50μm.