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. 2017 Jul 19;11(4):044105. doi: 10.1063/1.4995257

FIG. 4.

FIG. 4.

Experimental procedure for aptamer immobilization, ethanolamine blocking, and CRP detection. Note that the microfluidic chip could be re-used by using elution buffer. (a) 1st aptamer immobilization on the gate area of FET and removal of unbound 1st aptamer prior to detection; (b) blocking FET by ethanolamine and removal of unbound ethanolamine for 1 h; (c) CRP sample addition and removal of unbound CRP for 5 min; (d) 2nd aptamer addition and removal of unbound 2nd aptamer for 10 min; (e) elution buffer addition for CRP and removal of 2nd aptamer for the next test.