Figure 3.

Expression of FPR1 in colorectal cancer cell lines. The mRNA transcripts (a) and protein expression (b) of FPR1 in SW480 and HT29 were analyzed by agarose gel electrophoresis and western blotting, respectively. The full-length gels and blots were presented in Supplementary Fig. S4a and b. (c–f) Flow cytometry was used to confirm the distribution of FPR1 expression in these two cell lines. The cells were treated with or without membrane-penetration solution to detect the total protein expression (d,f) and membrane expression (c,e).