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. 2017 Jul 19;7:5901. doi: 10.1038/s41598-017-06142-x

Figure 3.

Figure 3

In vitro assessment of CSLPHNPs. Hormone-refractory metastatic prostate cancer PC-3 cells were treated with free drugs or CSLPHNPs for 72 hours. (A) Fluorescent/phase contrast images of PC-3 cells after incubation with CSLPHNPs containing Rhodamine B. (B) CSLPHNPs uptake assessed by fluorescent microscopy (A) was quantified by ImageJ. (C) Cytotoxicity of CSLPHNPs was assessed using the MTT assay. Columns, mean of three experiments performed in triplicate. Data is expressed as mean (n = 3) ±SE. *p < 0.05 versus control. D) PC-3 cells were treated for 48 h as indicated in figure and SK1 activity was measured by radiolabeling. Data is expressed as percentage relative to the control. Columns represent the mean of n = 3 ± SE. *p < 0.05 versus control.