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. 2017 Jul 19;7:5806. doi: 10.1038/s41598-017-05296-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition analysis of cell migration by a scratch assay in A549, MDA-MB-231 and B16F10 cells by treatment with Native BBM, BBM-NPs (with 1 μg/ml of mitomycin, a proliferation inhibition marker). (i) Microscopic picture of migrated cells after 28 hrs. (ii) Bar graph represents the number of cells migrated into the scratched area. Values are as mean ± SEM, n = 4, **p < 0.001, ***p < 0.0001 for Native BBM and BBM-NPs vs. control.