Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 19;7:5806. doi: 10.1038/s41598-017-05296-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Analysis of mitochondrial function and apoptosis by flow cytometry and expression of anti-apoptotic protein BCL-2 by western blot analysis. (a) A549 and MDA-MB-231 cells were treated with Native BBM and BBM-NPs (25 µM) for 48 hrs. Cells associated with mitochondrial function using Rhodamine 123 was analyzed by flow cytometry. Values are as mean ± SEM, n = 3, **p < 0.001, ***p < 0.0001 for Native BBM and BBM-NPs vs. control. (b) Induction of apoptosis in A549 and MDA-MB-231 cells treated with Native BBM/BBM-NPs (25 µM) for 48 hrs and percentage of apoptosis (early and late) was measured using Annexin V-FITC by flow cytometry. Values are as mean ± SEM, n = 3, **p < 0.001, ***p < 0.0001 for Native BBM and BBM-NPs vs. control. (c) Western blot analysis of anti-apoptotic protein BCL-2 associated with apoptosis in A549 and MDA-MB-231 cell lines following 25 µM BBM treatments (Native/NPs) for 48 hrs. β-actin serves as loading control.