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Comparison of the culture-dependent and culture-independent approaches. A natural whey culture (NWC) sample from La Praz, Switzerland was analyzed by plating. The slpH gene sequences were determined from 81 L. helveticus colonies by Sanger sequencing. The same NWC sample was examined using amplicon-based semiconductor sequencing without prior bacterial isolation. The stacked barplots show the relative abundance of each sequence type (ST) found in the sample for both methods.
