Table 3.
The developed strain typing approach was tested with a mixture of 10 L. helveticus strains.
| Assigned ST | N° of reads | Relative abundance (%) | Corresponding strain |
|---|---|---|---|
| ST13 | 826 (± 53) | 13.93 (± 0.59) | FAM8105 |
| ST15 | 732 (± 57) | 12.40 (± 1.12) | FAM21456 |
| ST10 | 462 (± 59) | 7.75 (± 0.08) | FAM13019 |
| ST11 | 423 (± 48) | 7.11 (± 0.18) | FAM22155 |
| ST8 | 365 (±31) | 6.14 (± 0.19) | FAM20575 |
| ST1 | 349 (± 42) | 5.85 (± 0.12) | FAM22076 |
| ST23 | 942 (± 193) | 15.73 (± 1.00) | FAM23235 |
| ST22 | 757 (± 112) | 12.68 (± 0.28) | FAM1213 |
| ST17 | 653 (± 137) | 12.89 (± 0.74) | FAM22330 |
| ST20 | 446 (± 46) | 7.51 (± 0.30) | FAM1450 |
Reads obtained after NGS were assigned to sequence types (STs) and the average relative abundance for each ST (± standard deviation, n = 3) was calculated. The corresponding strains were determined by comparing the read sequences to the genome sequences of the used strains.