Table 2.
The detection limit of the MLMA assay at both the DNA and bacterial levels.
| Type | Gene | LOD | |
|---|---|---|---|
| DNA level (ng/μL) | Bacterial level (CFU/mL) | ||
| V. cholerae | hlyA | 0.1 | 0.8 × 107 |
| CTX | 1.0 | 1.0 × 107 | |
| O1 V. cholerae | O1-rfbE | 1.0 | 1.2 × 108 |
| O139 V. cholerae | O139-wbfR | 1.0 | 1.5 × 108 |
| V. fluvialis | toxR | 0.1 | 0.9 × 107 |
| V. vulnificus | vvh | 0.1 | 0.7 × 107 |
| V. parahaemolyticus | toxR | 0.1 | 1.4 × 107 |
| tdh | 0.1 | 1.4 × 107 | |
| V. mimicus | vvh | 0.1 | 0.9 × 107 |
| V. alginolyticus | Collagenase | 0.1 | 1.1 × 107 |
| P. shigelloides | 23S rRNA | 0.1 | 1.2 × 108 |
| Aeromonas | 16S rRNA | 0.1 | 1.5 × 108 |