Figure 1.

Serum complement does not significantly impact B. bacteriovorus HD100 viabilities. Viability of (a) S. enterica, (b ) K. pneumoniae, (c) E. coli and (d) B. bacteriovorus HD100 in HEPES, 50% serum or 56 °C-treated serum (50%; complement inactivated) for up to 24 hours. p < 0.001 for a; p < 0.01 for b. n = 3. (e) Dot-blot results showing the serum possesses antibodies against each of the bacterial strains, particularly B. bacteriovorus HD100. Each spot contained between 2.5 × 10⁴ and 2.5 × 10⁶ bacteria per spot. The spots were probed using whole human serum and then with a peroxidase-conjugated anti-human goat IgG.