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Coomassie brilliant blue staining for total proteins. Semithin sections of both the control (A–E) and treated (F–J) embryos were obtained and stained with Coomassie brilliant blue. (A,F) Embryos grown for 6 DAP. (B,G) Embryos grown for 8 DAP. (C,H) Embryos grown for 10 DAP. (D,I) Embryos grown for 12 DAP. (E,J) Embryos grown for 14 DAP. Arrowheads indicate proteins accumulated in PSVs. Bar = 20 μm.
