Table 1.
Strains and plasmids used in this study.
| Plasmids or Strains | Relevant genotype or features | Source |
|---|---|---|
| E.coli | ||
| DH5a | Host for plasmid construction | TRANSGEN |
| BL21 | Host for overexpression | TRANSGEN |
| M. smegmatis mc 2 155 | ||
| M.sm/WT | M. smegmatis | This study |
| M.sm-pMV261 | mc2 155 with pmv261 | This study |
| M.sm-pMV261-ethr | mc2 155 with pmv261-Ms6441 | This study |
| M.sm-pMV261-dgc | mc2 155 with pmv261-Ms2196 | This study |
| M.sm-ethr::hyg | mc2 155 Ms6441 replaced by hyg | This study |
| M.sm-ethr::hyg-pMV261 | Msm Ms6441::hyg with pMV261 | This study |
| M.sm-ethr::hyg-pMV261-ethr | Msm Ms6441::hyg with pMV261:: Ms6441 | This study |
| M.sm-ethr::hyg-pMV261-dgc | Msm Ms6441::hyg with pMV261:: Ms2196 | This study |
| pET28a(+) | Kanr, T7 lac promoter, N-terminal His6 | This study |
| pET- Rv3855 | Rv3855 in Nde I-Eag I sites of pET28a | This study |
| pMV261 | Kanr, pAL5000 replicon | This study |
| pMV261- Ms6441 | Ms6441 in BamHI-HindIII site of pMV261 | This study |
| pMV261- Ms2196 | Ms2196 in BamHI-HindIII site of pMV261 | This study |