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. 2017 Jul 19;7:5929. doi: 10.1038/s41598-017-06092-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular analysis of the soil nitrogen cycle bacteria involved in the phases of fixation and denitrification, after treatment with the NC_20, NC_15, NC_10, and NC formulations. (A) Relative quantification of genes by qPCR; (B) Percentage distribution of each gene. The analyses were performed by qPCR of the following genes: nifH, narG, nirS, nirK, norB, and nosZ, at 15, 30, 60, 90, and 300 days after the first applications of the formulations.