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. 2017 Jul 19;7:5844. doi: 10.1038/s41598-017-06367-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Production of rhGALNS using the promoters tac and proU _mod, with two different E. coli strains: BL21(DE3) and SHuffle® T7. (A) Specific activity in the extracellular fraction. (B) Volumetric activity in the extracellular fraction. (C) Specific activity in the intracellular fraction. (D) Volumetric activity in the intracellular fraction. Enzyme activity was assessed 24 hours after induction (no induction was necessary in the case of the promoter proU _mod). A Student’s t-test was performed to assess significance, where (*) corresponds to a p-value < 0.05, (***) p-value < 0.001 and (****) p-value < 0.0001. Specific activities are expressed as U rhGALNS/(mg total protein) and volumetric activities as U/ml. (E) Western-blot of rhGALNS levels in the intracellular and extracellular fractions in the different studied conditions. All samples were normalized by the amount of total protein. Non-cropped versions of these figures can be found in the Supplementary Data.