Table 2.
Summary of the enzymatic rhGALNS activities obtained in this work after 24 hours of induction.
| Studied strains | Extracellular | Intracellular | ||
|---|---|---|---|---|
| U/mg total protein (% of improvement) | U/ml (% of improvement) | U/mg total protein (% of improvement) | U/ml (% of improvement) | |
| BL21(DE3)/pGEX-5X-GALNSopt | 0.08 ± 0.01 | 0.21 ± 0.04 | 0.01 ± 0.00 | 0.05 ± 0.01 |
| BL21(DE3)/pGEX-5X-GALNSopt 5% suc | 0.23 ± 0.03 (197%) | 0.67 ± 0.09 (216%) | 0.06 ± 0.02 (507%) | 0.14 ± 0.04 (219%) |
| BL21(DE3)/pGEX-5X-GALNSopt 10% suc | 0.20 ± 0.04 (149%) | 0.45 ± 0.00 (116%) | 0.01 ± 0.00 (0%) | 0.10 ± 0.03 (122%) |
| BL21(DE3)/pGEXproUmod | 0.67 ± 0.03 (751%) | 0.86 ± 0.04 (309%) | 0.08 ± 0.00 (695%) | 0.16 ± 0.01 (245%) |
| BL21(DE3)/pGEXproUmod 5% suc | 1.23 ± 0.16 (1,463%) | 1.20 ± 0.26 (470%) | 0.16 ± 0.01 (1,475%) | 0.20 ± 0.02 (335%) |
| BL21(DE3)/pGEXproUmod 10% suc | 0.62 ± 0.07 (682%) | 0.76 ± 0.09 (259%) | 0.07 ± 0.00 (589%) | 0.10 ± 0.00 (129%) |
| BL21(DE3)/pGEXproUmod 0.05 N NaCl | 0.97 ± 0.03 (1,134%) | 0.96 ± 0.03 (355%) | 0.14 ± 0.01 (1,234%) | 0.13 ± 0.01 (191%) |
| BL21(DE3)/pGEXosmY | 0.20 ± 0.04 (156%) | 0.34 ± 0.07 (63%) | 0.02 ± 0.00 (52%) | 0.05 ± 0.01 (8%) |
| BL21(DE3)/pGEXosmY 5% suc | 0.21 ± 0.04 (171%) | 0.31 ± 0.05 (48%) | 0.02 ± 0.01 (84%) | 0.04 ± 0.02 (−22%) |
| SHuffle/pGEX-5X-GALNSopt | 0.29 ± 0.03 (274%) | 0.23 ± 0.00 (7%) | 0.06 ± 0.01 (527%) | 0.04 ± 0.01 (−19%) |
| SHuffle/pGEXproUmod | 0.67 ± 0.01 (748%) | 0.50 ± 0.02 (138%) | 0.14 ± 0.00 (1,283%) | 0.07 ± 0.00 (65%) |
| SHuffle/pGEX-5X-GALNSopt 5% suc | 0.37 ± 0.04 (369%) | 0.31 ± 0.06 (48%) | 0.15 ± 0.02 (1,336%) | 0.07 ± 0.01 (47%) |
| SHuffle/pGEXproUmod 5% suc | 0.63 ± 0.15 (698%) | 0.52 ± 0.12 (147%) | 0.22 ± 0.07 (2,095%) | 0.10 ± 0.01 (118%) |
Values in bold correspond to statistically significant improvements using a t-student test with a p-value < 0.05.