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. 2017 Jul 1;24(7):716–724. doi: 10.5551/jat.36574

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2017 Japan Atherosclerosis Society

This article is distributed under the terms of the latest version of CC BY-NC-SA defined by the Creative Commons Attribution License.http://creativecommons.org/licenses/by-nc-sa/3.0/

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Fig. 4. — von Kossa staining of aortic rings cultured in normal Ca (1.8 mM) or high Ca (3.0 mM) under HPi (Pi 3.8 mM) with or without R-568. Culture in normal Ca (1.8 mM) under HPi (Pi 3.8 mM) is shown in the upper panels. Culture in high Ca (3.0 mM) under HPi (Pi 3.8 mM) is shown in the lower panels. Sections show diffuse severe calcification when cultured in all types of medium (A). Calcium content of aortic rings cultured in normal Ca (1.8 mM) or high Ca (3.0 mM) under HPi (Pi 3.8 mM) with or without R-568. Results are the average of at least 14 rings and represent an independent experiment (n = 14 rings obtained from eight rats) (B).