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. 2017 Jul 19;7:5922. doi: 10.1038/s41598-017-06397-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viable adipose tissue endothelial cells express a CD300f ligand. White adipose tissue (A–D) and lungs (D) from wild type (WT) and Il5 ^Tg mice were obtained, enzymatically digested and stained with viability and endothelial cells markers (A). Thereafter, the binding of CD300f-IgG1 Fc fusion protein to viable adipose tissue (B,C) and lung (D) endothelial cells was determined by flow cytometry. Data are representative of n = 4 independent experiments; *p < 0.05, ns- non-significant as analyzed by Student’s t-test (C).