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. 2017 Jul 19;7:5922. doi: 10.1038/s41598-017-06397-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cd300f ^−/− mice display age-related accumulation of eosinophils and macrophages and decreased adipose tissue weight. White adipose tissue (WAT) of young (2–4 months) and aged (5–8 months) wild type (WT) and Cd300f ^−/− mice were obtained and total eosinophils (A) and macrophages (B) per mg tissue were determined. Moreover, the weight of WATs from WT and Cd300f ^−/− mice was determined (C). In (D) a representative photograph of WAT size from WT and Cd300f ^−/− mice is shown. WT and Cd300f ^−/− mice were fed with high fat diet (HFD) for up to 18 weeks and weighed at the indicated time points (E). Following 18 weeks of HFD, mice were tested for glucose tolerance (F). Data in (A–C) are from n = 3, 2–3 mice per time point; in (D) data are representative of on one out of eight; in (E–F) data are from n = 3 using 10 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001 as analyzed by Student’s t-test (A–C) and two-way ANOVA followed by Tukey post-hoc test (E,F).