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. 2017 Jul 19;7:5925. doi: 10.1038/s41598-017-06132-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CEP55 overexpression promoted pancreatic cancer (PANC) cells aggressiveness in vitro. (a) Expression of CEP55 protein as detected by Western blot analysis in SW1990 and Capan-1 cells upon transfection with CEP55 or CPE55 short interfering RNAs (siRNAs). GAPDH was used as the loading control. (b) MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay of SW1990 and Capan-1 cell growth curves following CEP55 or CEP55 siRNAs transfection. (c) Representative micrographs (left panel) and quantification (right panel) of crystal violet-stained SW1990 and Capan-1 cell colonies after 14 day of colony formation. (d) Wound-healing assay (left panel) and quantification (right panel) of the wound distance to assess SW1990 and Capan-1 cell migration after transfection with CEP55 or CEP55 siRNAs. (e) Representative micrographs (left panel) and quantification of SW1990 and Capan-1 cell invasion in a Transwell matrix penetration assay. The quantification of the invaded cells is represented by the mean of three independent experiments. Bars represent the mean ± SD of three independent experiments. *P < 0.05.