Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 19;7:5925. doi: 10.1038/s41598-017-06132-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The nuclear factor kappaB (NF-κB) pathway contributed to CEP55-mediated aggressiveness and tumourigenicity of pancreatic cancer (PANC) cells in vitro and in vivo. (a) The protein expression of IκBα transfected into CEP55-overexpressing PANC cells is detected by western blotting assay. (b) MTT assay of CEP55-overexpressing SW1990 and Capan-1 cells transduced with IκBα or JSH-23. (c) Quantification of crystal violet-stained colonies of CEP55-overexpressing SW1990 and Capan-1 cells transduced with IκBα or JSH-23 after 14 days of colony formation. (d) Wound-healing assay assessment of CEP55-overexpressing SW1990 and Capan-1 cell migration after transfection with IκBα or JSH-23. (e) Quantification of CEP55-overexpressing SW1990 and Capan-1 cell invasion in a Transwell matrix penetration assay. The quantification of invaded cells represents the mean of three independent experiments. (f) Subcutaneous tumour xenograft model in nude mice; representative images of the tumours formed by CEP55-overexpressing Capan-1 cells transfected with IκBα (left panel, n = 5). Growth curves for tumour formation after implantation of CEP55-overexpressing Capan-1 cells transfected with IκBα (right panel). Bars represent the mean ± SD of three independent experiments. *P < 0.05.