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. 2017 Jul 19;7:5941. doi: 10.1038/s41598-017-05734-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of unesterified cholesterol in wild-type and calreticulin-deficient subcellular fractions. (A) Immunoblot analysis of wild-type and Calr ^−/− subcellular fractions probed with anti-calnexin and anti-GAPDH antibodies. Representative of 3 biological replicates. (B) Distribution of unesterified cholesterol in the subcellular fractions of wild-type and Calr ^−/− cells. Representative of 3 biological replicates. The images of (A) shown are cropped. The full-length gel/blots are shown in Fig. S11. See “Experimental Procedures” for additional details.