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. 2017 Jul 19;7:5892. doi: 10.1038/s41598-017-06364-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Barrier integrity of trophoblast cell lines based on trans-epithelial electrical resistance (TEER). (a) Influence of initial seeding density of BeWo b30, ACH-3P, Jeg-3 and JAR placental cells on electrical resistance on day 5 post-seeding. (b) Increase of electrical resistance of BeWo b30, ACH-3P, Jeg-3 and JAR placental cell barriers on day 1, 3, 5 and 7 post-seeding. Data points are presented as mean values ± SD for n = 6 and expressed as absolute values. Values on day 0 correspond to blank membrane values of acellular controls.