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. 2017 Jul 19;7:5892. doi: 10.1038/s41598-017-06364-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Secretion of hCG of four different placental cell lines. (a) Apical and basal secretion of hCG of BeWo, ACH-3P, Jeg-3 and JAR placental transwell cultures at day 5 post seeding (n = 3). (b) Response to forskolin treatment of hCG secretion of trophoblast derived cell lines BeWo, ACH-3P, Jeg-3 and JAR placental cell lines in 24-well microtiter plates at day 7 post seeding. Cells were stimulated with 50 µM forskolin at day 5. Data points are mean values ± SD for n = 3. Data is normalized to cell numbers and expressed as fold increase of the untreated controls.