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. 2017 Jul 19;7:5791. doi: 10.1038/s41598-017-06055-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neighbor-joining phylogenetic tree of the ten most abundant OTUs in the analyzed kitchen sponges, as retrieved by pyrosequencing of 16S rRNA gene amplicon libraries. The relative abundance (percentage of the total sequence dataset) and the detection frequency (number of sponges where they were detected) are given in parenthesis after the OTU number. The most similar reference sequences retrieved by BLAST and EzTaxon alignment (type strains only) were included in the tree, followed by the corresponding accession numbers. Red circles indicate risk group 2 organisms, according to the German Technical Rule for Biological Agents No. 466 (TRBA 466³¹). Numbers at the nodes indicate percentage values of 1000 bootstrap re–samplings (only percentages ≥ 50 are shown). Scale bar represents substitution rate per nucleotide position.