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. 2017 Jul 19;7:5814. doi: 10.1038/s41598-017-06261-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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POL stimulated the maturation of DCs in immunized C57BL/6 mice. 3 days after the 3^rd vaccination, the splenocytes of immunized mice were prepared and stained for DCs analysis by flow cytometry. The samples were stained with anti-mouse CD11c-FITC/CD40-PE mAbs or CD11c-FITC/CD80-PE mAbs for DCs maturation. The CD11c⁺CD40⁺ cells or CD11c⁺CD80⁺ cells were counted relatively to total CD11c⁺ cells.