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. 2017 Jul 19;7:5870. doi: 10.1038/s41598-017-05038-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular localisation of TFCP2 and ASIC2a in the epileptic rats’ CA1 regions and glucose-deficient cells. (a) Double immunofluorescence labelling for TFCP2 and ASIC2a in the epileptic rats’ CA1 regions during acute and latent post-seizure phases. (b) Cellular localisation of TFCP2 and ASIC2a expression in PC12 cells grown for 24 h in high-glucose media (control), low-glucose media, no-glucose media, and STF-31 (10 µM)-loaded media. Proteins were probed with anti-ASIC2a (green) and anti-TFCP2 (red) antibodies. Nuclei were counterstained with DAPI (blue). Scale bars = 20 μm. Abbreviations, ASIC2a: acid-sensing ion channel 2a; TFCP2: transcription factor CP2; DAPI: 4′,6-diamidino-2-phenylindole.