Table 3.
Limit of detection (LOD) and limit of quantification (LOQ) of the pure compounds analyzed.
| Compound | Linear regression | R2 | LOD (ng/mL) | LOQ (ng/mL) | IS |
|---|---|---|---|---|---|
| Adrenic acid | |||||
| Ent-7(RS)-7-F2t-dihomo-IsoP | y = 0.0044 x − 0.0339 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| 17(RS)-F2t-dihomo-IsoP | y = 0.0065 x − 0.0639 | 0.998 | 7.81 | 15.63 | d4-15-F2t isoP |
| Arachidonic acid | |||||
| 2,3-dinor-15-F2t-IsoP | y = 0.0079 x − 0.0558 | 0.998 | 0.98 | 1.95 | d4-15-F2t isoP |
| Ent-15(RS)-2,3-dinor-5,6-dihydro-15-F2t-IsoP | y = 0.0079 x − 0.0747 | 0.998 | 3.91 | 7.81 | d4-15-F2t isoP |
| 15-epi-15-F2t-IsoP | y = 0.0024 x − 0.0183 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| 15-F2t-IsoP | y = 0.0027 x − 0.0208 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| 5-F2t-IsoP | y = 0.0032 x − 0.0240 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| 5-epi-5-F2t-IsoP | y = 0.0042 x − 0.0348 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| Alpha-Linolenic acid | |||||
| Ent-16-epi-16-F1t-PhytoP | y = 0.0136 x − 0.1393 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| Ent-16-F1t-PhytoP | y = 0.0133 x − 0.1109 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| 9-F1t-PhytoP | y = 0.0166 x − 0.1316 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| 9-epi-9-F1t-PhytoP | y = 0.0160 x − 0.1293 | 0.998 | 1.95 | 3.91 | d4-15-F2t isoP |
| Eicosapentaenoic acid | |||||
| 8-F3t-IsoP | y = 0.0042 x − 0.0314 | 0.998 | 0.98 | 1.95 | d4-15-F2t isoP |
| 8-epi-8-F3t-IsoP | y = 0.0035 x − 0.0299 | 0.998 | 0.98 | 1.95 | d4-15-F2t isoP |
| 5-F3t-IsoP | y = 0.0018 x − 0.0186 | 0.998 | 3.91 | 7.81 | d4-15-F2t isoP |
| 5-epi-5-F3t-IsoP | y = 0.0012 x − 0.0111 | 0.998 | 3.91 | 7.81 | d4-15-F2t isoP |
| Docosahexaenoic acid | |||||
| 10-F4t-NeuroP | y = 0.0017 x − 0.0117 | 0.998 | 0.49 | 0.98 | d4-10-F4t-NeuroP |
| 10-epi-10-F4t-NeuroP | y = 0.0023 x − 0.0162 | 0.998 | 0.98 | 1.95 | d4-10-epi-10-F4t-NeuroP |
| 14(RS)-14-F4t-NeuroP | y = 6.2450.10−5 x − 6.7300.10−4 | 0.998 | 15.63 | 31.25 | d4-10-epi-10-F4t-NeuroP |
| 4(RS)-F4t-NeuroP | y = 1.8386.10−4 x − 0.0019 | 0.998 | 7.81 | 15.63 | d4-4(RS)-F4t-NeuroP |
The deuterated form of IsoP and NeuroP were used as internal standards to determine the LOD and LOQ of the analytes. IsoP: isoprostane; NeuroP: neuroprostane.