Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 19;7:5853. doi: 10.1038/s41598-017-05829-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Rnq1 and Sup35 physically interact. (A) ThT kinetic experiments monitor the polymerization of amyloid via enhanced fluorescent emission. Unseeded Sup35 (blue line) polymerizes and aggregates after approximately 17 hours, as does Sup35 incubated with Rnq1 monomer alone (green line). The addition of Rnq1 fibers (orange line) reduces the lag time for Sup35 polymerization to approximately 12.5 hours. Curves represent data from three experiments. (B) Sup35 was immobilized on resin and utilized as bait for a [RNQ ⁺] trap assay. Cell lysates were incubated with the resin, washed with buffer, and eluted. The presence of trapped, untagged Rnq1 was detected via western blot with anti-Rnq1 antibody. Lanes shown are the unbound (UB) fraction, the final wash fraction and the first two elution fractions. “Wash*” indicates an intermediate wash fraction. Blots are representative images from three independent experiments.