Skip to main content
. 2017 Jul 19;7:5853. doi: 10.1038/s41598-017-05829-5

Figure 4.

Figure 4

Sup35 mutations can restore interaction with Rnq1-Q298R. (A) Candidates from a suppressor screen to identify Sup35 mutants that can rescue the [PSI +] induction defect associated with rnq1-Q298R. These mutants enhanced [PSI +] formation in the rnq1-Q298R genetic background relative to WT SP5. Horizontal white bars separate non-adjacent spottings from the same plate. Complete spottings of screen candidates appear in Supplementary Figure S2. (B) The indicated mutations were cloned into SUP35 for further testing.