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. 2017 Jul 19;7:5917. doi: 10.1038/s41598-017-06256-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison schematic of FJ223607-like (Plasmid 9-like) IncN plasmids (publicly available; this study), and their geographic origin/dates of isolation. Plasmid sequence names in red are those from this study, derived from PacBio data and closed (ecol_517, ecol_656) or incomplete plasmid structures (ecol_516, ecol_736) derived from Illumina data. Aligned bars adjacent to plasmid names represent plasmid sequences: light grey denotes regions with 100% sequence identity; black represents nucleotide diversity between sequences; and thin lines represent indels. Coding sequences are represented by fat arrows below individual sequence bars and are colour-coded as per the colour key. The inset schematic describing genetic variation between sequences depicts examples of evolutionary events identified: (a) single nucleotide level change, (b) small indels (≤100 bp), (c) large indels (>100 bp), (d) recombination events.