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. 2017 Jul 19;7:5801. doi: 10.1038/s41598-017-06374-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of Pcdh19 induces clustering of neurons in the cerebral cortex. (a–d) pNeuroD-Cre driven expression of GFP without (a,b) or with Pcdh19b-FLAG (c,d) in cortical neurons. DNA transfer was performed by in utero electroporation at E12.5. (a) GFP-labeled neurons were distributed in the cortical plate. (b) Fluorescence intensities along the red line indicated in the rightmost image in a. Arrows indicate GFP-positive cell bodies, and asterisk indicates a neuronal process along the line. (c) GFP-labeled neurons expressing Pcdh19-FLAG formed clusters in the cortical plate. (d) Fluorescence intensities along the red line indicated in the rightmost image in (c). Arrows indicate the GFP-positive cell bodies, and asterisk indicates a neuronal process along the line. Scale bar, 50 μm.